| Details of the hOPT set-up (a, b and c), consisting of an infinity-corrected detection objective (OLd), an emission filter (Fd), a tube lens (TL), and a diaphragm (I) coupled to an electron-multiplied CCD camera, an excitation source (LED1) and an excitation filter (Fi) and a reference white light (LED2) illuminating the sample through a diffusor (D) (shown only in (c)). Translation of the sample and rotation is done through controlled step motors (d). The samples (S) are either attached to a capillary for single specimen measurements, or stacked inside a capillary for high-throughput. Images are taken in air, using the bath (B) to control the humidity and environment. |
