(Left) Schematic dorsal view of the mouse brain. The holographic mesoscope allows targeted photostimulation in a 1×1 mm FOV (magenta square) combined with simultaneous mesoscale imaging across a 5×5 mm imaging FOV (dotted white). (Right) Example of a 2p mesoscale image over a large field-of-view in a transgenic mouse. The magenta square indicates the accessible holographic FOV within an example mesoscale imaging FOV of 2.4 mm × 2.4 mm recorded at 4.5 Hz frame rate. Credit: UC Berkeley. |
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