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| 3D live-cell super-resolution imaging with eSRRF: Mitochondria dynamics observed in U2OS cells expressing TOM20-Halo, loaded with the fluorescent marker JF54, with a multi-focus microscope are super-resolved by 3D eSRRF processing of the dataset. This allows a super-resolved volumetric view of 20 x 20 x 3.6 μm3 at a rate of ~1Hz in a living cell. |
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