| Comparison between confocal (left) and STED imaging (right) using continuous wave and pulsed illumination. The top row demonstrates STED imaging on fluorescent latex beads (diameter 20 nm). The second example (middle row) shows syntaxin clusters in the membrane of PC12 cells while the bottom row shows the distribution of the protein synaptophysin in the endosomes of mammalian cells (Images recorded by B Harke, K Willig, G Donnert, Max Planck Institute for Biophysical Chemistry, Göttingen, top to bottom). Credit: B Harke, K Willig and G Donnert. |
