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| Electronic transitions involved in stimulated emission depletion (STED) microscopy (left). Exponentially decreasing fluorescence emission as a function of STED laser power (right). The resolution d of the STED microscope is given by an extended version of Abbe's equation (inset). Here, I is the maximum intensity found at the doughnut crest and Is is the saturation intensity at which the fluorescence drops to 1/e of its original value. Is is characteristic of the dye and the wavelength in use and is typically 10–100 MW/cm2. Credit: Max Planck Institute for Biophysical Chemistry. |
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