Fluorescence Filter Sets

12 Apr 2022

Fluorescence imaging utilises different optical filters to excite a fluorescent dye or protein and direct the resulting emitted wavelengths to the detector.

At Knight Optical, we are pleased to announce our new range of fluorescence filter sets. Each set comprises of an excitation bandpass filter, an emission bandpass filter, and a dichroic beamsplitter, that have been carefully designed to work alongside certain fluorophores whilst ensuring any unwanted wavelengths are blocked. These hard coated fluorescence filters have superior transmission at the excitation and emission wavelengths, whilst providing excellent blocking at the crossover.

In a typical fluorescence microscopy setup, the excitation filter narrows the amount of light that is directed on to the dichroic beamsplitter, set at 45°, and consequently on to the sample, containing the fluorophore. The emitted wavelengths transmit through the dichroic beamsplitter then on to the emission filter which further refines the wavelengths ensuring that any unwanted light does not reach the detector.

Our stock catalogue currently includes the following fluorescence filter sets, with provided information on the centre wavelengths for the bandpass filters and the cut-on wavelength for the dichroic beamsplitter.

Each set is suitable for multiple fluorophores, further information for each set can be found on product information sheets found their stock page.