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Product Announcement

Fluorescence Filter Sets

12 Apr 2022

Fluorescence imaging utilises different optical filters to excite a fluorescent dye or protein and direct the resulting emitted wavelengths to the detector.

At Knight Optical, we are pleased to announce our new range of fluorescence filter sets. Each set comprises of an excitation bandpass filter, an emission bandpass filter, and a dichroic beamsplitter, that have been carefully designed to work alongside certain fluorophores whilst ensuring any unwanted wavelengths are blocked. These hard coated fluorescence filters have superior transmission at the excitation and emission wavelengths, whilst providing excellent blocking at the crossover.

In a typical fluorescence microscopy setup, the excitation filter narrows the amount of light that is directed on to the dichroic beamsplitter, set at 45°, and consequently on to the sample, containing the fluorophore. The emitted wavelengths transmit through the dichroic beamsplitter then on to the emission filter which further refines the wavelengths ensuring that any unwanted light does not reach the detector.

Our stock catalogue currently includes the following fluorescence filter sets, with provided information on the centre wavelengths for the bandpass filters and the cut-on wavelength for the dichroic beamsplitter.

DAPI fluorescence filter set

340FFL25-ST

Excitation

340nm

 

TRITC fluorescence filter set

525FFL25-ST

Excitation

525nm

Emission

450nm

 

Emission

595nm

Dichroic cut-on

390nm

 

Dichroic cut-on

565nm

 

   

 

 

   

CFP fluorescence filter set

440FFL25-ST

Excitation

440nm

 

ROX fluorescence filter set

560FFL25-ST

Excitation

560nm

Emission

480nm

 

Emission

645nm

Dichroic cut-on

455nm

 

Dichroic cut-on

595nm

 

   

 

 

   

FITC fluorescence filter set

475FFL25-ST

Excitation

475nm

 

CY5 fluorescence filter set

605FFL25-ST

Excitation

605nm

Emission

535nm

 

Emission

695nm

Dichroic cut-on

505nm

 

Dichroic cut-on

650nm

 

   

 

 

   

YFP fluorescence filter set

495FFL25-ST

Excitation

495nm

 

 

   

Emission

545nm

 

 

   

Dichroic cut-on

525nm

 

 

   

Each set is suitable for multiple fluorophores, further information for each set can be found on product information sheets found their stock page.

CONTACT DETAILS
Knight Optical Ltd
Roebuck Business Park
Harrietsham
Kent
ME17 1AB
United Kingdom
Tel: 44 (0)1622 859444
Fax: 44 (0)1622 859 555
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