Dichroic colour filters for Fluorescence Microscopy and Spectroscopy

14 Feb 2017

Need to measure the fluorescent properties of a component with your Spectrometer but you feel like something’s missing?

Maybe it’s Knight Optical range of Stock Dichroic Filters, ideal for use within Fluorescence Microscopy and Spectroscopy.

For Fluorescence Microscopy and Spectroscopy, dichroic filters are used as beam splitters within the microscope system. They are used as an edge filter at a tilted angle of incidence (typically 45°) to direct the illumination of an excitation frequency towards the sample of interest, and then at an analyser to reject the same excitation frequency but passes a particular emission frequency.

Dichroic filters offer superior contrast between transmitted and reflected wavelengths; having negligible absorption. Having a dielectric coating they are perfect for high power applications and they do not show the strong fluorescence of certain glass filters.

We hold a massive inventory of Bandpass, Long pass, Short pass, Band-blocking and Colour-correction filters.

Stock sizes of 12.5mm and 25mm diameter; and 50x50mm squares x 1mmthk.
425mmdia stock sheets are kept in stock for your specific requirements.
Custom shapes and sizes at short notice, in most cases turned around within 1 week.
Coated on Borofloat substrates for excessive thermal shock applications.
Alternative thickness substrates available.

.Typical specs:

Material: Borosilicate or equivalent, Sodalime
Diameter Tolerance: +0.0/-0.20 mm
Length/Width Tolerance: +/- 0.25mm
Thickness Tolerance: +/-0.20mm
Wavelength Range: 300nm-1200nm dependant on type
Wavelength Accuracy: +/- 5nm
Transmission: Tmax=95% dependant on type and substrate

All our Dichroic Filters are fully inspected on their quality in our ISO 9001 certified, state-of-the-art Metrology laboratory, using our Varian Cary 5000 spectrophotometers which are fitted with a UMA. This allows us to work to the highest QA standards and meet the tolerance specifications on these precision components.

Contact our technical sales team to discover how Knight Optical’s high quality Dichroic Filters and superior service can improve your instrumentation and supply chain experience.

UK, Europe, Asia & RoW: E-Mail info@knightoptical.co.uk Tel +44 (0)1622 859444

USA & Canada: E-Mail usasales@knightoptical.com Tel +001 401-583-7846