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| Two-photon microscopy captures axonal segments in a living brain, enabling researchers to quantify structural changes in specialized areas under noninvasive low-intensity repetitive transcranial magnetic stimulation (rTMS). A zoomed-out survey of the brain area (A) shows complex networks of neurons, visible via fluorescent marker. Insets (B and C) show specialized axonal endings (termed “boutons”) where synapses form. TB axon: “terminaux boutons” are short protrusions from the axon shaft typically connecting neurons in a local area. EPB axon: “en passant boutons” are small bead-like structures along axons typically connecting distal regions. Images courtesy of B. Fulopova (University of Queensland). |
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