Supercontinuum Source for STED Microscopy Nov 6, 2009

Available at a fraction of the cost of traditional methods, the Fianium source SC450-PP-HE will enable many more users to utilise this exciting technique.

Stimulated emission depletion (STED) fluorescence is a powerful method in microscopy allowing sub-diffraction-limited resolution to be achieved. STED microscopy has typically been implemented using a large-frame Ti:sapphire laser and an Optical Parametric Amplifier to offer the range of spectral components required in the visible. Such a system has cost in the region of $500k.

The SC450-PP-HE laser is based on Fianium’s patent pending variable repetition rate supercontinuum fiber laser technology. Minor modifications to the core technology of the SC450-PP laser system has enabled the delivery of supercontinuum with enhanced energy spectral density (>1nJ/nm – a 20-fold increase over conventional system) required for STED illumination.

Further developments of the SC450-PP-HE now provide the capability to have STED at wavelengths below 500nm and excitation down to 400nm, enabling further expansion of the method to cover a wider variety of fluorescent dyes. Furthermore, scaling of both the energy spectral density and pulse repetition rate will enable enhanced resolution and scan speeds respectively.

For more information and a detailed application note on STED please visit www.fianium.com, call +44(0)2380 458776 or email info@fianium.com.

About Fianium
Fianium is the leading manufacturer of high-performance ultrafast fiber lasers for applications in bio-medical, materials processing and scientific research. The company was founded in 2003 with objective to challenge the dominance of ultrafast DPSS and pioneered the development of new generation of fiber lasers that combine the advantages of semiconductor technology with the maturity of specialty optical fibers. Fianium’s ultrafast fiber lasers deliver superior performance, reliability and usability at a lower total cost of ownership compared with conventional solid state lasers.

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